SIGNUP SHEET on googledocs
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This section is a collection of presentations of students made as a part of their assignments. Topics are picked by you from a list. The presentation time is 15 minutes and scoring is based on
S. No. | Date | Presenter | Technique | Reference |
---|---|---|---|---|
1 | 14-Sep-2012 | A. Pathak | Atomic force microscopy for imaging | Kuznetsova (2007) Atomic force microsopy probing of cell elasticity. Micron. 38: 824 |
2 | 14-Sep-2012 | Ketakee Ghate | Fluorescence activated cell sorting (FACS) | Freed NE et al. (2008) A simple screen to identify promoters conferring high levels of phenotypic noise. PLoS Genet. 4(12):e1000307. |
3 | 21-Sep-2012 | V. Krithika | Microfluidics for bacterial chemotaxis | Mao et al. (2003) A sensitive, versatile microfluidic assay for bacterial chemotaxis. PNAS USA 100: 5449-5454. |
4 | 21-Sep-2012 | Roopali Pradhan | Fluorecence recovery after photobleaching (FRAP) to estimate diffusion coefficients of molecules | Mika JT et al. (2011) Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells.PLoS One. 6(9):e25664. |
5 | 12-Oct-2012 | 2D gel electrophoresis for quantification of protein abundance | Fey & Larsen (2001) 2D or not 2D. Curr. Op. Chem. Biol. 5:p26 | |
6 | 12-Oct-2012 | DNA sequencinging by Sanger dye termination and denaturation. | Chen et al. (2010) DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device. Lab on a Chip. 10:p1153. | |
7 | 12-Oct-2012 | DNA nanopore sequencing | (a) Cherf et al. (2012) Automated forward and reverse ratcheting of DNA in a nanopore at 5-Angstrom precision. Nat. Biotech. 30, p344-348. | |
8 | 12-Oct-2012 | DNA nanopore sequencing | (b) Manrao et al. (2012) Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase. Nat. Biotech.30, 349–353 | |
9 | Atomic force microscopy for DNA flexibility measurement | Wiggins et al. (2006) High flexibility of DNA on short length scales probed by atomic force microscopy. Nat. Nanotech. 1: 137 - 141 | ||
10 | DNA devices | Modi et al. (2009) A DNA nanomachine that maps spatial and temporal pH changes inside living cells. Nature Nanotech. 4: 325 | ||
11 | DNA origami and motors | Wickham et al. (2012) A DNA-based molecular motor that can navigate a network of tracks. 7: 169. | ||
12 | Dynamic light scattering | Y. Georgalisa et al. (2012) Light scattering studies on Ficoll PM70 solutions reveal two distinct diffusive modes. J. Colloid & Interface Science. Volume 386, Issue 1, Pages 141–147 | ||
13 | Surface plasmon resonance in biology | Kim D. et al. (2012) Heptameric Targeting Ligands against EGFR and HER2 with High Stability and Avidity.PLoS One. 7(8):e43077. | ||
14 | Two-photon microscopy for tissue imaging | Johannssen HC, Helmchen F. (2012) Two-photon imaging of spinal cord cellular networks. Exp Neurol. | ||
15 | Confocal laser scanning microscopy | Cheng et al. (1999) Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method. Biophys J. 76(2): 606–617. | ||
16 | 4pi Microscopy | Hüve et al. (2008) 4Pi Microscopy of the Nuclear Pore Complex. Biophys J. 95(2): 877-885. | ||
17 | Fluorescence loss in photobleaching (FLIP) to estimate molecular mobility | Judd et al. (2003) Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in Caulobacter. PNAS 100(14):8235-40. | ||
18 | Single plane illumination microscopy (SPIM) | |||
19 | Dynamic light scattering for in vitro protein measurements | |||
20 | Photoactivation in diffusion measurements | Bakshi S et al. (2011) Subdiffraction-limit study of Kaede diffusion and spatial distribution in live Escherichia coli.Biophys J.101(10):2535-44. | ||
21 | STORM nanoscopy | |||
22 | STED nanoscopy | |||
23 | Second harmonic generation and CARS | |||
24 | Magnetic tweezers to study molecular motors | Kim & Saleh (2009) A high-resolution magnetic tweezer for single-molecule measurements Saleh1,2,Nucleic Acids Res. 37(20): e136. | ||
25 | Optical tweezers | Moffit et al. (2008) Recent advances in optical tweezers. Ann. Rev. Biochem. 77: 205-228. | ||
26 | Optical tweezers to study forces in constant force mode | Chen et al. (2009) Stretching Submicron Biomolecules with Constant-Force Axial Optical Tweezers. Biophys J. 96: 4710-4708 | ||
27 | Cell traction force microscopy | |||
28 | Laser nanosurgery | |||
29 | Femtosecond resolved absorption spectroscopy | |||
30 | Isothermal calorimetry (ITC) | |||
31 | ITC to measure enzyme kinetics | |||
32 | Patch clamp | |||
33 | Patch clamp for measuring single ion-channel potentials | |||
34 | Scanning electron microscopy (SEM) | |||
35 | Scanning tunneling microscope (STM) | |||
36 | Fluorescence resonance energy transfer (FRET) | Violin et al. (2003) A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 161(5):899-909. |