This section is a collection of presentations of students made as a part of their assignments. Topics are picked by you from a list. The presentation time is 15 minutes and scoring is based on (a) content, (b) clarity and (c) the question-answer session. The talk itself must have the following components (i) introduction to the technique, (ii) physical principle/algorithm/maths, and (iii) biological application.
S. No. | Date | Topic | Reference | Student presenting |
1 | 2D gel electrophoresis for quantification of protein abundance | Fey & Larsen (2001) 2D or not 2D. Curr. Op. Chem. Biol. 5:p26 | ||
2 | DNA sequencinging by Sanger dye termination and denaturation. | Chen et al. (2010) DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device. Lab on a Chip. 10:p1153. | ||
3 | DNA nanopore sequencing | (a) Cherf et al. (2012) Automated forward and reverse ratcheting of DNA in a nanopore at 5-Angstrom precision. Nat. Biotech. 30, p344-348. | ||
4 | DNA nanopore sequencing | (b) Manrao et al. (2012) Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase. Nat. Biotech.30, 349–353 | ||
5 | Atomic force microscopy for imaging | Kuznetsova (2007) Atomic force microsopy probing of cell elasticity. Micron. 38: 824 | A. Pathak | |
6 | Atomic force microscopy for DNA flexibility measurement | Wiggins et al. (2006) High flexibility of DNA on short length scales probed by atomic force microscopy. Nat. Nanotech. 1: 137 - 141 | ||
7 | DNA devices | Modi et al. (2009) A DNA nanomachine that maps spatial and temporal pH changes inside living cells. Nature Nanotech. 4: 325 | ||
8 | DNA origami and motors | Wickham et al. (2012) A DNA-based molecular motor that can navigate a network of tracks. 7: 169. | ||
9 | Fluorescence activated cell sorting (FACS) | Ketakee Ghate | ||
10 | Dynamic light scattering | |||
11 | Surface plasmon resonance in biology | |||
12 | Two-photon microscopy for tissue imaging | |||
13 | Confocal laser scanning microscopy | |||
14 | 4pi Microscopy | |||
15 | Fluorescence loss in photobleaching (FLIP) to estimate molecular mobility | |||
16 | Single plane illumination microscopy (SPIM) | |||
17 | Fluorecence recovery after photobleaching (FRAP) to estimate diffusion coefficients of molecules | Roopali Pradhan | ||
18 | Dynamic light scattering for in vitro protein measurements | |||
19 | Photoactivation in diffusion measurements | |||
18 | STORM nanoscopy | |||
19 | STED nanoscopy | |||
20 | Second harmonic generation and CARS | |||
21 | Magnetic tweezers to study molecular motors | |||
22 | Optical tweezers | Moffit et al. (2008) Recent advances in optical tweezers. Ann. Rev. Biochem. 77: 205-228. | ||
23 | Optical tweezers to study forces in constant force mode | Chen et al. (2009) Stretching Submicron Biomolecules with Constant-Force Axial Optical Tweezers. Biophys J. 96: 4710-4708 | ||
24 | Cell traction force microscopy | |||
25 | Laser nanosurgery | |||
26 | Femtosecond resolved absorption spectroscopy | |||
27 | Isothermal calorimetry (ITC) | Shubhankar Kulkarni | ||
28 | ITC to measure enzyme kinetics | |||
29 | Patch clamp | |||
30 | Patch clamp for measuring single ion-channel potentials | |||
31 | Scanning electron microscopy (SEM) | |||
32 | Scanning tunneling microscope (STM) | |||
33 | Microfluidics for bacterial chemotaxis | Mao et al. (2003) A sensitive, versatile microfluidic assay for bacterial chemotaxis. PNAS USA 100: 5449-5454. |