[Course site] [IISER Pune]

Biophysical Techniques

This section is a collection of presentations of students made as a part of their assignments. Topics are picked by you from a list. The presentation time is 15 minutes and scoring is based on (a) content, (b) clarity and (c) the question-answer session. The talk itself must have the following components (i) introduction to the technique, (ii) physical principle/algorithm/maths, and (iii) biological application.

S. No. Date Topic Reference Student presenting
1 2D gel electrophoresis for quantification of protein abundance Fey & Larsen (2001) 2D or not 2D. Curr. Op. Chem. Biol. 5:p26
2 DNA sequencinging by Sanger dye termination and denaturation. Chen et al. (2010) DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device. Lab on a Chip. 10:p1153.
3 DNA nanopore sequencing (a) Cherf et al. (2012) Automated forward and reverse ratcheting of DNA in a nanopore at 5-Angstrom precision. Nat. Biotech. 30, p344-348.
4 DNA nanopore sequencing (b) Manrao et al. (2012) Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase. Nat. Biotech.30, 349–353
5 Atomic force microscopy for imaging Kuznetsova (2007) Atomic force microsopy probing of cell elasticity. Micron. 38: 824 A. Pathak
6 Atomic force microscopy for DNA flexibility measurement Wiggins et al. (2006) High flexibility of DNA on short length scales probed by atomic force microscopy. Nat. Nanotech. 1: 137 - 141
7 DNA devices Modi et al. (2009) A DNA nanomachine that maps spatial and temporal pH changes inside living cells. Nature Nanotech. 4: 325
8 DNA origami and motors Wickham et al. (2012) A DNA-based molecular motor that can navigate a network of tracks. 7: 169.
9 Fluorescence activated cell sorting (FACS) Ketakee Ghate
10 Dynamic light scattering
11 Surface plasmon resonance in biology
12 Two-photon microscopy for tissue imaging
13 Confocal laser scanning microscopy
14 4pi Microscopy
15 Fluorescence loss in photobleaching (FLIP) to estimate molecular mobility
16 Single plane illumination microscopy (SPIM)
17 Fluorecence recovery after photobleaching (FRAP) to estimate diffusion coefficients of molecules Roopali Pradhan
18 Dynamic light scattering for in vitro protein measurements
19 Photoactivation in diffusion measurements
18 STORM nanoscopy
19 STED nanoscopy
20 Second harmonic generation and CARS
21 Magnetic tweezers to study molecular motors
22 Optical tweezers Moffit et al. (2008) Recent advances in optical tweezers. Ann. Rev. Biochem. 77: 205-228.
23 Optical tweezers to study forces in constant force mode Chen et al. (2009) Stretching Submicron Biomolecules with Constant-Force Axial Optical Tweezers. Biophys J. 96: 4710-4708
24 Cell traction force microscopy
25 Laser nanosurgery
26 Femtosecond resolved absorption spectroscopy
27 Isothermal calorimetry (ITC) Shubhankar Kulkarni
28 ITC to measure enzyme kinetics
29 Patch clamp
30 Patch clamp for measuring single ion-channel potentials
31 Scanning electron microscopy (SEM)
32 Scanning tunneling microscope (STM)
33 Microfluidics for bacterial chemotaxis Mao et al. (2003) A sensitive, versatile microfluidic assay for bacterial chemotaxis. PNAS USA 100: 5449-5454.