Biophysics II: bio435 Laboratory

Experimental and theoretical exercises to test concepts in physical biology of molecules, cells and tissues

1. Crowding and enzyme kinetics
   Reference:
   1. Olsen et al. (2007) Effects of osmolytes on hexokinase kinetics combined with macromolecular crowding. Comp. Biochem. & Physiol. 148: 339. [PDF]
   2. Jarvis et al. (1990) "Macromolecular Crowding"
   3. Hexokinase kinetics with PEG as a crowdant Reid & Rand(1996)
   4. Fitting product time-course data for enzyme velocity estimates Duggleby (1985)
   5. Sigma Hexokinase Assay
2. FRAP in bacteria- Measuring in vivo mobility of proteins
3. Measuring the effect of crowding on diffusive motility of aggregates in vitro
4. Spatial patterns by diffusion and aggregation of bacteria in culture- a paradigm for development