Biology

Dr. Maddika SubbaReddy
Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad

Protein-protein interactions are intrinsic to virtually every cellular process such as Cell survival, proliferation, cell cycle control, transcription, translation, DNA replication, signal transduction, splicing, secretion and metabolism. Our lab is interested in mapping the functional protein interaction networks in various cellular processes. One of the major areas in the lab is focused on dissecting phosphatase biology based on their interactome. Here, we aim to systematically identify and functionally characterize the network of interacting proteins of all the available phosphatases in regulation of eukaryotic cellular processes.

By using biochemical purification followed by LC-MS/MS based proteomic analysis we identified the associated protein complexes of 142 phosphatases and developed a highly complex network of phosphatase interactome built on 6597 high confident interactions. We assigned novel cellular functions to different phosphatases based on their interacting partners. A detailed interactome analysis along with our work on some of the novel functional phosphatase interactions would be discussed.